IL18-CART

CAR T Cells Secreting IL18 Augment Antitumor Immunity and Increase T Cell Proliferation and Costimulation

  

http://biorxiv.org/content/early/2017/02/26/111260

Research highlight

• Human IL18-CART displays dramatic proliferation and severe GVHD in xenograft model.

• Recombinant IL18 costimulated IFNG secretion and proliferation of anti-CD3 beads treated T cells.

• Crispr/Cas9 targeting shows IL18 driven proliferation requires both TCR and IL18R.

• sTH18 (synthetic T Helper cell expressing IL18): CD4 T cells help is involved in IL18 mediated costimulation.

• TCR- CD19-IL18 CART confers superior proliferation and antitumor activity, without induction of GVHD effect.

• APACHE (Antigen Propelled Activation of Cytokine Helper Ensemble) T cells achieve CAR driven IL18 production by eliminating endogenous TCR.